Composite

Part:BBa_K3316009:Design

Designed by: Luana Langlois   Group: iGEM19_Western_Canada   (2019-10-12)


Laccase-SpyTag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 442
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To help purify the fusion protein, we added a 6-His tag at the N-terminus of the protein. The choice of linker is to minimize constraints in conformation in either portion of the fusion protein product due to sterics.


Source

The FliC 5'UTR and peptide sequence was derived from the Escherichia coli K12 genome. The SpyTag moiety is endogenous to Streptococcus pyogenes. The laccase enzyme is endogenous to Escherichia coli K12.

References